Germination studies of sunflower immature embryos that were preserved in refrigeration and plants acclimatization

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Comportamiento de la contaminación y la germinación, en tres genotipos a los diferentes tiempos de refrigeración de las semillas.
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Published: Aug 20, 2007
Keywords:
germination, immature embryos, sunflower

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Ana Julia Rodríguez Mansito
Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”
Yanisbel Sánchez Rodríguez
Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”
Dayamí Pérez Hernández
Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”
Norma Marrero Granado
Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”
Guillermo Brito Iglesias
Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”

Abstract

Immature embryos culture gives the possibility to obtain plant lines in a relatively short period of time with the reduction of generational intervals for obtaining select sunflower materials. In occasions, it is necessary to preserve materials for some period of time and their advantages or disadvantages must be known. This paper studied the effect of immature seed preservation at low temperature (4 oC) on contamination, embryo germination, in vitro plantlets growth and adaptation of different genotypes to environment conditions. This study will contribute to adjust the methodology for utilization in genetic improvement programs. Immature seeds of three genotypes (maintained in the head or in plastic flasks) at fifteen days after pollination beginning and different period of times in domestic refrigerator were used. Embryos were cultured in MS medium with half of nutrients. Contamination and germinating percentage were calculated and plantlets high were measure, at seven days of culture. Obtained plantlets were adapted to environmental conditions. It was observed that short refrigeration time of seeds (seven days) stimulated plantlet growth. Plantlets from all studied genotypes were obtained at forty days of preservation of immature seeds at low temperature and they were adapted to soil conditions.

Article Details

How to Cite
Germination studies of sunflower immature embryos that were preserved in refrigeration and plants acclimatization. (2007). Agrotecnia De Cuba, 31(2), 86-91. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/729
Issue
Vol. 31 No. 2 (2007): May-August
Section
Original Articles
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How to Cite

Germination studies of sunflower immature embryos that were preserved in refrigeration and plants acclimatization. (2007). Agrotecnia De Cuba, 31(2), 86-91. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/729

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